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Multiplication-stimulating activity for chicken embryo fibroblasts from rat liver cell conditioned medium: A family of small polypeptides

✍ Scribed by Norman C. Dulak; Howard M. Temin

Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
784 KB
Volume
81
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

A partially purified multiplication‐stimulating activity for chicken embryo fibroblasts in cell culture was isolated from rat liver cell conditioned medium (see preceding paper, Dulak and Temin, 1973). It has been analyzed by isoelectric focusing and by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Multiplication‐stimulating activity resided in a family of at least four polypeptides which were similar in apparent molecular size, but different in electrical charge. These polypeptides have a specific activity of about 50,000 with respect to serum. One of them has been purified on a small scale to apparent homogeneity in a sodium dodecyl sulfate polyacrylamide gel.

📜 SIMILAR VOLUMES

A partially purified polypeptide fractio
A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts
✍ Norman C. Dulak; Howard M. Temin 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 719 KB

## Abstract A polypeptide fraction with multiplication‐stimulating activity for chicken and rat embryo fibroblasts was partially purified from serum‐free medium conditioned by the growth of a line of rat liver cells. The specific multiplication‐stimulating activity of this fraction was 27,000 times

Large scale purification and further cha
Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity
✍ Norman C. Dulak; Yuen W. Shing 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 945 KB

## Abstract The application of roller‐bottle cell culture techniques and a relatively simple purification scheme has led to the isolation of milligram quantities of a polypeptide cell multiplication stimulating activity (MSA) from Buffalo rat liver cell conditioned medium. We have characterized the

Purified multiplication-stimulating acti
Purified multiplication-stimulating activity from rat liver cell conditioned medium: Comparison of biological activities with calf serum, insulin, and somatomedin
✍ Gary L. Smith; Howard M. Temin 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 1000 KB

## Abstract Multiplication‐stimulating activity (MSA) for chicken embryo fibroblasts was purified from serum‐free medium conditioned by the growth of a line of rat liver cells (CRL), The biological activities of purified CRL MSA for chicken embryo fibroblasts were compared with those of calf serum

The partial purification from calf serum
The partial purification from calf serum of a fraction with multiplication-stimulating activity for chicken fibroblasts in cell culture and with non-suppressible insulin-like activity
✍ Robert W. Pierson Jr.; Howard M. Temin 📂 Article 📅 1972 🏛 John Wiley and Sons 🌐 English ⚖ 853 KB

## Abstract A multiplication‐stimulating activity for chicken embryo fibroblasts has been purified approximately 6000‐fold from calf serum by a three step procedure. The partially purified multiplication‐stimulating activity stimulated DNA synthesis, mitosis, and growth of cultured chicken embryo f