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A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts

✍ Scribed by Norman C. Dulak; Howard M. Temin

Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
719 KB
Volume
81
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

A polypeptide fraction with multiplication‐stimulating activity for chicken and rat embryo fibroblasts was partially purified from serum‐free medium conditioned by the growth of a line of rat liver cells. The specific multiplication‐stimulating activity of this fraction was 27,000 times that of serum. The rat liver cell multiplication‐stimulating activity had a molecular weight of approximately 10,000 daltons and was inactivated by mercaptoethanol and dithiothreitol. It had sulfation factor and non‐suppressible insulin‐like activities, but did not have anti‐trypsin activity. The rat liver cell multiplication‐stimulating activity resembled both multiplication‐stimulating activity from calf serum and somatomedin.

📜 SIMILAR VOLUMES

Multiplication-stimulating activity for
Multiplication-stimulating activity for chicken embryo fibroblasts from rat liver cell conditioned medium: A family of small polypeptides
✍ Norman C. Dulak; Howard M. Temin 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 784 KB

## Abstract A partially purified multiplication‐stimulating activity for chicken embryo fibroblasts in cell culture was isolated from rat liver cell conditioned medium (see preceding paper, Dulak and Temin, 1973). It has been analyzed by isoelectric focusing and by polyacrylamide gel electrophoresi

Purified multiplication-stimulating acti
Purified multiplication-stimulating activity from rat liver cell conditioned medium: Comparison of biological activities with calf serum, insulin, and somatomedin
✍ Gary L. Smith; Howard M. Temin 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 1000 KB

## Abstract Multiplication‐stimulating activity (MSA) for chicken embryo fibroblasts was purified from serum‐free medium conditioned by the growth of a line of rat liver cells (CRL), The biological activities of purified CRL MSA for chicken embryo fibroblasts were compared with those of calf serum

The partial purification from calf serum
The partial purification from calf serum of a fraction with multiplication-stimulating activity for chicken fibroblasts in cell culture and with non-suppressible insulin-like activity
✍ Robert W. Pierson Jr.; Howard M. Temin 📂 Article 📅 1972 🏛 John Wiley and Sons 🌐 English ⚖ 853 KB

## Abstract A multiplication‐stimulating activity for chicken embryo fibroblasts has been purified approximately 6000‐fold from calf serum by a three step procedure. The partially purified multiplication‐stimulating activity stimulated DNA synthesis, mitosis, and growth of cultured chicken embryo f