✦ LIBER ✦

Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity

✍ Scribed by Norman C. Dulak; Yuen W. Shing

Publisher: John Wiley and Sons
Year: 1977
Tongue: English
Weight: 945 KB
Volume: 90
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040900115

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✦ Synopsis

Abstract

The application of roller‐bottle cell culture techniques and a relatively simple purification scheme has led to the isolation of milligram quantities of a polypeptide cell multiplication stimulating activity (MSA) from Buffalo rat liver cell conditioned medium. We have characterized the apparently homogeneous MSA with respect to its biological activity, its N‐terminal amino acid residue, and its amino acid composition, and have tested the MSA for growth‐promoting activity in a number of cell types.

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Multiplication-stimulating activity for

Multiplication-stimulating activity for chicken embryo fibroblasts from rat liver cell conditioned medium: A family of small polypeptides

✍ Norman C. Dulak; Howard M. Temin 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 784 KB

## Abstract A partially purified multiplication‐stimulating activity for chicken embryo fibroblasts in cell culture was isolated from rat liver cell conditioned medium (see preceding paper, Dulak and Temin, 1973). It has been analyzed by isoelectric focusing and by polyacrylamide gel electrophoresi

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A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts

✍ Norman C. Dulak; Howard M. Temin 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 719 KB

## Abstract A polypeptide fraction with multiplication‐stimulating activity for chicken and rat embryo fibroblasts was partially purified from serum‐free medium conditioned by the growth of a line of rat liver cells. The specific multiplication‐stimulating activity of this fraction was 27,000 times

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Purified multiplication-stimulating activity from rat liver cell conditioned medium: Comparison of biological activities with calf serum, insulin, and somatomedin

✍ Gary L. Smith; Howard M. Temin 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 1000 KB

## Abstract Multiplication‐stimulating activity (MSA) for chicken embryo fibroblasts was purified from serum‐free medium conditioned by the growth of a line of rat liver cells (CRL), The biological activities of purified CRL MSA for chicken embryo fibroblasts were compared with those of calf serum