Multiplex polymerase chain reaction: A practical approach
β Scribed by P. Markoulatos; N. Siafakas; M. Moncany
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 27 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
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A probabilistic approach to the kinetics of the polymerase chain reaction (PCR) is developed. The approach treats the primer extension step of PCR as a microscopic Markov process in which the molecules of deoxy-nucleoside triphosphate (dNTP) are bound to the 3 end of the primer strand one at a time.
Pathology practice is increasingly augmented with molecular tests for improved diagnostics and patient management. The polymerase chain reaction (PCR) is foremost amongst these techniques. This review explains the principles of PCR and the methodological factors that contribute to a successful assay
## Abstract A multiplex polymerase chain reaction (PCR) assay that is capable of detecting and typing six serotypes of respiratory adenovirus (Ad) was developed, using multiple sets of typeβspecific primers. The detection of each different serotype depended on distinguishing different numbers and s