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The polymerase chain reaction: Development of a practical illustrating its use

✍ Scribed by Zoe Smith; C David O'Connor


Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
388 KB
Volume
20
Category
Article
ISSN
0307-4412

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The development of a quadriplex PCR method with amplification of HCMV in a single-step procedure using primers taken from four different regions of the viral genome is described. Different concentrations of dNTPs and MgCl 2 were assayed in order to optimize the constitution of the buffer for the mul