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Measurement of Gene Expression by Multiplex Competitive Polymerase Chain Reaction

✍ Scribed by M.J. Apostolakos; W.H.T. Schuermann; M.W. Frampton; M.J. Utell; J.C. Willey


Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
715 KB
Volume
213
Category
Article
ISSN
0003-2697

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We have established competitive reverse transcriptase polymerase chain reaction (RT-PCR) assay for the quantification of MDR1 mRNA encoding P-glycoprotein (P-gp) by analyzing leukemia sublines of MOLT-3 with various expression of MDR1. The expression was quantified by simultaneous RT-PCR of cellular