𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression

✍ Scribed by Marianne Jörgensen; Maja Bévort; Thuri S. Kledal; Brian V. Hansen; Marlene Dalgaard; Henrik Leffers


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
247 KB
Volume
20
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Quantitative analysis of human multidrug
✍ Hiroyuki Kobayashi; Yuzuru Takemura; Hayato Miyachi; Masaki Kawabata; Shigehisa 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 124 KB 👁 2 views

We have established competitive reverse transcriptase polymerase chain reaction (RT-PCR) assay for the quantification of MDR1 mRNA encoding P-glycoprotein (P-gp) by analyzing leukemia sublines of MOLT-3 with various expression of MDR1. The expression was quantified by simultaneous RT-PCR of cellular

A Polymerase Chain Reaction-Based Method
✍ M.J. Morales; D.I. Gottlieb 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 583 KB

Transcription in higher eukaryotes is often studied by the use of transient transfection assays. These experiments are performed by cloning putative cis-acting transcriptional elements (i.e., a promoter or enhancer) with a reporter gene that codes for a protein not expressed by the target cells. Alt