Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression
✍ Scribed by Marianne Jörgensen; Maja Bévort; Thuri S. Kledal; Brian V. Hansen; Marlene Dalgaard; Henrik Leffers
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 247 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0173-0835
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Transcription in higher eukaryotes is often studied by the use of transient transfection assays. These experiments are performed by cloning putative cis-acting transcriptional elements (i.e., a promoter or enhancer) with a reporter gene that codes for a protein not expressed by the target cells. Alt