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Quantitative Polymerase Chain Reaction Assay for Aggrecan and Link Protein Gene Expression in Cartilage

✍ Scribed by P. Re; W.B. Valhmu; M. Vostrejs; D.S. Howell; S.G. Fischer; A. Ratcliffe


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
488 KB
Volume
225
Category
Article
ISSN
0003-2697

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We have established competitive reverse transcriptase polymerase chain reaction (RT-PCR) assay for the quantification of MDR1 mRNA encoding P-glycoprotein (P-gp) by analyzing leukemia sublines of MOLT-3 with various expression of MDR1. The expression was quantified by simultaneous RT-PCR of cellular