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Multicenter assessment of HIV-1 RNA quantitation in semen in the CREAThE network

✍ Scribed by Christophe Pasquier; Corinne Andreutti; Evelyne Bertrand; Alionka Bostan; Thomas Bourlet; Irene Molina; Zehava Grossman; Philippe Halfon; Marianne Leruez-Ville; Margrethe Lüneborg-Nielsen; Carmen Mar; Anne-Geneviève Marcelin; Catherine Roussel-Ronserail; Marie-Paule Schmitt; Sepehr Tabrizi; Maria Vourliotis; Louis Bujan

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
79 KB
Volume
84
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

Detection of HIV‐1 RNA in semen is used commonly to determine the safety of semen processing procedures before assisted reproductive technology (ART). Using two panels of prepared semen samples containing HIV‐1 the performances of protocols from 14 centers have been compared. No false‐positive results were detected but false‐negative results were frequent when the concentration was below 500 HIV‐1 RNA copies/ml of seminal plasma. Frequency of HIV‐1 RNA detection was higher on seminal cells than on seminal plasma. Assays (or protocols) for quantifying HIV‐1 RNA in semen performed less well than standardized blood plasma assays. The HIV load in seminal plasma could be a useful marker of the risk of sexual transmission of the virus. Its use as a marker of global HAART efficiency in the HIV reservoir needs further study. Standardized assays are required for detection and measurement of HIV‐1 RNA in semen samples. J. Med. Virol. 84:183–187, 2012. © 2011 Wiley Periodicals, Inc.

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