✦ LIBER ✦

Quantitation of hiv-1 rna in blood cells of arc and aids patients

✍ Scribed by Robert Solomon; James Thompson; David Gillespie

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 721 KB
Volume: 3
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860030505

No coin nor oath required. For personal study only.

✦ Synopsis

Peripheral blood mononuclear cells from HIV-1 -infected persons were mixed with 5 M guanidine thiocyanate, and HIV-1 -specific probes were hybridized with target RNA directly in the lysate. No RNA purification was needed. Hybrids were purified by repeated capture on superparamagnetic beads coated

📜 SIMILAR VOLUMES

Quantitation of hepatitis C virus RNA in

Quantitation of hepatitis C virus RNA in peripheral blood mononuclear cells in HCV-monoinfection and HIV/HCV-coinfection

✍ Aarthi Chary; Mark A. Winters; Robert Eisen; Thomas H. Knight; David M. Asmuth; 📂 Article 📅 2012 🏛 John Wiley and Sons 🌐 English ⚖ 148 KB

## Abstract Peripheral blood mononuclear cells (PBMCs) represent an extrahepatic hepatitis C virus (HCV) reservoir, the significance of which is unclear due to limited studies and varying test methodologies. In this study, a commercial viral load assay for measuring cell‐associated PBMC HCV RNA was

Electronic Transduction of HIV-1 Drug Re

Electronic Transduction of HIV-1 Drug Resistance in AIDS Patients

✍ Lital Alfonta; Immanuel Blumenzweig; Maya Zayats; Lea Baraz; Moshe Kotler; Itama 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 181 KB

Multicenter assessment of HIV-1 RNA quan

Multicenter assessment of HIV-1 RNA quantitation in semen in the CREAThE network

✍ Christophe Pasquier; Corinne Andreutti; Evelyne Bertrand; Alionka Bostan; Thomas 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 79 KB

## Abstract Detection of HIV‐1 RNA in semen is used commonly to determine the safety of semen processing procedures before assisted reproductive technology (ART). Using two panels of prepared semen samples containing HIV‐1 the performances of protocols from 14 centers have been compared. No false‐p

Detection of HIV-RNA-positive monocytes

Detection of HIV-RNA-positive monocytes in peripheral blood of HIV-positive patients by simultaneous flow cytometric analysis of intracellular HIV RNA and cellular immunophenotype

✍ Bruce K. Patterson; Victoria L. Mosiman; Luis Cantarero; Manohar Furtado; Mondir 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 124 KB 👁 3 views

Determinations of plasma HIV viral RNA copy numbers help to define the kinetics of HIV-1 infection in vivo and to monitor antiretroviral therapy. However, questions remain regarding the identity of various infected cell types contributing to this free virus pool and to the in vivo lifecycle of HIV d

HIV antigen in brains of patients with A

HIV antigen in brains of patients with AIDS

✍ Dana H. Gabuzda; Raymond A. Sobel 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 115 KB

Antiretroviral genotypic resistance in p

Antiretroviral genotypic resistance in plasma RNA and whole blood DNA in HIV-1 infected patients failing HAART

✍ Annalisa Saracino; Nicola Gianotti; Marianna Marangi; Donatella C. Cibelli; Andr 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 197 KB

## Abstract The extent to which HIV‐1 proviral DNA mutations cause clinically relevant antiretroviral resistance is still controversial. Paired plasma HIV‐1 RNA and whole blood DNA were compared in patients failing HAART to investigate if the additional knowledge of archived mutations could improve