Variations in lateral asymmetry of constitutive heterochromatin were studied in 30 normal individuals with reference to the chromosomal regions 1q12, 9q12, 15p11, 16q12 and Yq12. The technique consisted of growing human lymphocytes for one cell cycle in BrdU, staining with 33258 Hoechst, exposing th
Morphologic variability of human chromosomes: Polymorphism of constitutive heterochromatin
β Scribed by P. K. Ghosh; Indera P. Singh
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 484 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0340-6717
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β¦ Synopsis
Polymorphism of constitutive heterochromatin has been studied in a series of 30 normal individuals. A high frequency of C-band variants were observed. Twenty-six of the 30 individuals studied had at least one polymorphic variant of the C band. A total of 42 variants were recorded which were predominately localized near the centromeric heterochromatin block of chromosome 9 (26.19%), chromosome 16 (19.05%), and chromosome 1 (16.66%). These results are discussed together with the findings revealed by different studies.
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In cultured amniotic fluid cells a mediocentric chromosome 9 appeared to be completely deficient in constitutive heterochromatin when stained with distamycin A and DAPI. In addition, this deficient chromosome was found in a blood cell culture from the father. Both the father and the child after birt
Linear measurement of blocks of constitutive heterochromatin and the euchromatin portion 1q-h in three members of a family was used to study the dependence of the size of C blocks on the degree of chromosomal contraction. The results demonstrate that the size of heterochromatin portions decrease reg