Variations in lateral asymmetry of constitutive heterochromatin were studied in 30 normal individuals with reference to the chromosomal regions 1q12, 9q12, 15p11, 16q12 and Yq12. The technique consisted of growing human lymphocytes for one cell cycle in BrdU, staining with 33258 Hoechst, exposing th
Length of human constitutive heterochromatin in relation to chromosomal contraction
✍ Scribed by Petr Balíček; Jan Žižka; Hana Skalská
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 520 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0340-6717
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✦ Synopsis
Linear measurement of blocks of constitutive heterochromatin and the euchromatin portion 1q-h in three members of a family was used to study the dependence of the size of C blocks on the degree of chromosomal contraction. The results demonstrate that the size of heterochromatin portions decrease regularly with an increases of the degree of euchromatin contraction. The dependence was found to be linear, except for mitoses with an extremely high or low degree of contraction. The finding was used for the development of a new method of evaluation of constitutive heterochromatin.
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