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Complete deficiency of constitutive heterochromatin on a human chromosome 9

✍ Scribed by C. H. C. M. Buys; J. M. M. Ypma; W. L. Gouw

Publisher: Springer
Year: 1979
Tongue: English
Weight: 227 KB
Volume: 49
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00277634

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✦ Synopsis

In cultured amniotic fluid cells a mediocentric chromosome 9 appeared to be completely deficient in constitutive heterochromatin when stained with distamycin A and DAPI. In addition, this deficient chromosome was found in a blood cell culture from the father. Both the father and the child after birth were phenotypically normal. Evidently, a considerable heterozygotic deficit of chromosome 9 heterochromatin can be tolerated without affecting the phenotype. The heterochromatin defect was also shown by G11-staining. Distamycin A-DAPI staining is highly reproducible and is recommended as a fluorescent alternative to often less successful G11-methods for the detection of heteromorphism of chromosome 9.

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