✦ LIBER ✦

Heterogeneity of human chromosome 9 constitutive heterochromatin as revealed by sequential distamycin A/DAPI staining and C-banding

✍ Scribed by C. H. C. M. Buys; W. L. Gouw; J. A. M. Blenkers; C. H. Dalen

Publisher: Springer
Year: 1981
Tongue: English
Weight: 338 KB
Volume: 57
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00271162

No coin nor oath required. For personal study only.

✦ Synopsis

Distamycin A/DAPI staining and sequential C-banding of human lymphocyte chromosomes reveals the regular occurrence of differentially staining subfractions of chromosome 9 constitutive heterochromatin. These subfractions are regionally organized as two subsegments: a distal one, which fluoresces brightly with DAPI after preincubation with distamycin A and a proximal one, which stains intensely with Giemsa after sequential C-banding. Observations are presented that indicate an occasionally independent genetic behavior of these heterochromatin subfractions.