𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Monte Carlo simulation of multiple attack mechanism of β-amylase-catalyzed reaction

✍ Scribed by Hiroshi Nakatani

Publisher
Wiley (John Wiley & Sons)
Year
1997
Tongue
English
Weight
118 KB
Volume
42
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

b-Amylase (EC 3.2.1.2) produces maltose (dimer) from the nonreducing ends of a-1,4 glucosidic bonds of substrates like maltooligosaccharides, amylose, and amylopectin. The enzyme releases several maltose molecules from a single enzyme-substrate complex without dissociation by multiple or repetitive attack containing many branching reaction paths. The Monte Carlo method was applied to the simulation of the b-amylase-catalyzed reaction including the multiple attack mechanism. The simulation starts from a single enzyme molecule and a finite number of substrate molecules. The selection of the substrate by the enzyme and degree of multiple attack proceeds by random numbers produced from a computer. The simulation was carried out until the whole substrate and the intermediate molecules were consumed. The simulated data were compared with experimental data of sweet potato b-amylase using heptamer, octamer, nanomer, and 11-mer as substrates. The only adjustable parameter for oddnumbered substrates was the probability of multiple attack, while an additional adjustable parameter (a correction factor due to low reactivity of tetramer) was needed for even-numbered substrates.

📜 SIMILAR VOLUMES

Monte Carlo simulation of multiple attac
Monte Carlo simulation of multiple attack mechanism of α-amylase
✍ Hiroshi Nakatani 📂 Article 📅 1998 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 379 KB

Porcine pancreatic a-amylase (EC 3.2. I . I ) produces short maltooligosaccharides from a single enzyme-substrate complex without dissociation by multiple or repetitive attack. Multiple attack is caused by relative sliding ofthe enzyme along the product chain ofthe enzyme-product complex without dis

Constant number Monte Carlo simulation o
Constant number Monte Carlo simulation of population balances with multiple growth mechanisms
✍ Samira Khalili; Yulan Lin; Antonios Armaou; Themis Matsoukas 📂 Article 📅 2010 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 378 KB

## Abstract We present a complete simulation scheme for particulate processes based on the constant number Monte Carlo methodology. Specifically, the proposed scheme can be applied towards the solution of population balances that include nucleation, coagulation and surface deposition, coupled to ch

A Monte Carlo simulation of the depolyme
A Monte Carlo simulation of the depolymerization of linear homopolymers by endo-enzymes exhibiting random-attack probability and single-attack mechanism: Application to the (1→3),(1→4)-β-D-glucan/endo-(1→3),(1→4)-β-D-glucanase system
✍ José V. Carbonell; Luis Izquierdo; José M. Sendra; Paloma Manzanares 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 192 KB 👁 2 views

A Monte Carlo simulation of the depolymerization of linear homopolymers by specific endo-enzymes exhibiting random-attack probability and a single-attack mechanism has been developed. The program simulates the ''real'' depolymerization versus time of a polydisperse sample of substrate by a specific