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Molecular weight estimation of Triton X-100 solubilized proteins by polyacrylamide gel electrophoresis

โœ Scribed by Vincent J. Hearing; Walter G. Klingler; Thomas M. Ekel; Paul M. Montague

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
632 KB
Volume
72
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A polyacrylamide gel elec&ophoresis system is described which employs the nonionic detergent T&on X-100 as a protein solvent. Aside from the obvious advantages of this detergent over ionic detergents and urea in the preservation of protein structure and function, it is demonstrated that the ancillary benefits of gel electrophoresis.

i.e., molecular size and charge determination, are possible.

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