A method employing stable isotope labeling and fast-atom bombardment (FAB) tandem mass spectrometry has been developed to directly assess events of biosynthesis and metabolism of arachidonic acid containing phospholipid molecular species by cells carried in culture. Mast cells, cultured with [(13)C]
Molecular species analysis of arachidonate containing glycerophosphocholines by tandem mass spectrometry
โ Scribed by Kathleen Kayganich; Robert C. Murphy
- Book ID
- 108044600
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 963 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1044-0305
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
To illustrate tandem mass spectrometry (MS/MS) as an alternative to GC/MS for the analysis of trichothecenes in solid and liquid cultures, diacetoxyscerpenol (DAS) levels were measured by GC/MS and by three MS/MS protocols. GC/MS had the lowest detection limit and better precision than the MS/MS pro
A convenient universal and fast mass spectrometrical method designed for the molecular species analysis of natural lipids is described. In contrast to the commonly employed procedures the method does not require chemical or enzymatic treatment and does not include chromatographic steps. The method r