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Molecular species analysis of lipids by metastable ion mass spectrometry

✍ Scribed by S.G. Batrakov; V.L. Sadovskaya; B.V. Rozynov; L.D. Bergelson

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
931 KB
Volume
33
Category
Article
ISSN
0009-3084

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✦ Synopsis

A convenient universal and fast mass spectrometrical method designed for the molecular species analysis of natural lipids is described. In contrast to the commonly employed procedures the method does not require chemical or enzymatic treatment and does not include chromatographic steps. The method relies on the recognition of ions characteristic of individual molecular species in the mass spectrum of a particular lipid fraction, that is accomplished on the basis of metastable ion spectra. The etficiency of this approach is demonstrated with a variety of natural lipids: triglycerides, glycerophospholipids, sphingomyelin and ornithinolipids. The advantages and limitations of the method as well as possible further developments are discussed.

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Herein we describe a rapid, simple, and reliable method for the quantitative analysis and molecular species fingerprinting of triacylglycerides (TAG) directly from chloroform extracts of biological samples. Previous attempts at direct TAG quantitation by positive-ion electrospray ionization mass spe