## Abstract We previously isolated an acyclovir (ACV)‐resistant herpes simplex virus type 1 (HSV‐1), strain TAR, from a child with Wiskott‐Aldrich syndrome. An acyclovir‐sensitive HSV‐1, strain TAS, had been isolated from the same patient before the isolation of HSV‐1 TAR. The TK protein of ACV‐sen
Molecular genetic analysis of thymidine kinase of the herpes simplex virus type 1
✍ Scribed by A. A. Guskova; A. V. Zagurny; M. Yu. Skoblov; A. V. Baranova; V. L. Andronova; N. K. Yankovsky; G. A. Galegov; Yu. S. Skoblov
- Publisher
- SP MAIK Nauka/Interperiodica
- Year
- 2005
- Tongue
- English
- Weight
- 94 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0026-8933
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A simple method to determine the in vitro catalytic turnover constant of several substrates of herpes simplex virus type 1 thymidine kinase is presented in this study. The method is based on a continuous spectroscopic enzyme-coupled assay and allows one to monitor the herpes simplex virus type 1 thy
## Abstract Isozyme analyses have been carried out to investigate the sites of integration of the herpes simplex type 2 (HSV‐2) thymidine kinase (TK) gene in biochemically transformed human [HeLa(BU25)/HSV‐2–6 Cl 4] cells. Extracts were prepared from He La (BU25)/HSV‐2–6 Cl 4 cells and from human—m