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Molecular Cloning and Heterologous Expression of a True Lipase in Pichia pastoris Isolated via a Metagenomic Approach

✍ Scribed by Zheng, Jianhua; Liu, Liguo; Liu, Cuina; Jin, Qi


Book ID
120202485
Publisher
S. Karger AG
Year
2012
Tongue
English
Weight
552 KB
Volume
22
Category
Article
ISSN
1464-1801

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## Abstract An ovalbumin gene was cloned from Chinese quail (__Coturnix coturnix__) oviduct by RT‐PCR and then inserted into the __P. pastoris__ genome under the control of the methanol inducible 5β€² alcohol oxidase (AOX) promoter. The recombinant __P. pastoris__ strain was demonstrated to be able t