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Cloning, sequence analysis and heterologous expression in Pichia pastoris of a gene encoding a thermostable cellobiose dehydrogenase from Myriococcum thermophilum

✍ Scribed by Marcel Zámocký; Christina Schümann; Christoph Sygmund; John O’Callaghan; Alan D.W. Dobson; Roland Ludwig; Dietmar Haltrich; Clemens K. Peterbauer


Book ID
116828779
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
333 KB
Volume
59
Category
Article
ISSN
1046-5928

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## Abstract An ovalbumin gene was cloned from Chinese quail (__Coturnix coturnix__) oviduct by RT‐PCR and then inserted into the __P. pastoris__ genome under the control of the methanol inducible 5′ alcohol oxidase (AOX) promoter. The recombinant __P. pastoris__ strain was demonstrated to be able t