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Cloning of a gene encoding Na+-coupled phosphate symporter from Pichia pastoris and use of its promoter for expression of heterologous protein

✍ Scribed by Hongweon Lee; Jiyeon Hong; Jungoh Ahn; Myongsoo Park; Eungyo Lee; Euisung Choi


Book ID
118472332
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
58 KB
Volume
131
Category
Article
ISSN
0168-1656

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## Abstract An ovalbumin gene was cloned from Chinese quail (__Coturnix coturnix__) oviduct by RT‐PCR and then inserted into the __P. pastoris__ genome under the control of the methanol inducible 5β€² alcohol oxidase (AOX) promoter. The recombinant __P. pastoris__ strain was demonstrated to be able t