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Molecular cloning of a laccase gene from Ganoderma lucidum and heterologous expression in Pichia pastoris

โœ Scribed by You, Lin-Feng; Liu, Zhi-Ming; Lin, Jun-Fang; Guo, Li-Qiong; Huang, Xun-Liu; Yang, Hai-Xing


Book ID
120406738
Publisher
John Wiley and Sons
Year
2013
Tongue
English
Weight
536 KB
Volume
54
Category
Article
ISSN
0233-111X

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## Abstract An ovalbumin gene was cloned from Chinese quail (__Coturnix coturnix__) oviduct by RTโ€PCR and then inserted into the __P. pastoris__ genome under the control of the methanol inducible 5โ€ฒ alcohol oxidase (AOX) promoter. The recombinant __P. pastoris__ strain was demonstrated to be able t