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Cloning of a Laccase Gene from a Novel BasidiomyceteTrametessp. 420 and Its Heterologous Expression inPichia pastoris

โœ Scribed by Yu-zhi Hong; Hong-min Zhou; Xiao-ming Tu; Jian-feng Li; Ya-zhong Xiao


Publisher
Springer
Year
2007
Tongue
English
Weight
105 KB
Volume
54
Category
Article
ISSN
0343-8651

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Cloning of a novel ovalbumin gene from q
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## Abstract An ovalbumin gene was cloned from Chinese quail (__Coturnix coturnix__) oviduct by RTโ€PCR and then inserted into the __P. pastoris__ genome under the control of the methanol inducible 5โ€ฒ alcohol oxidase (AOX) promoter. The recombinant __P. pastoris__ strain was demonstrated to be able t