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Cloning and characterization of the inulinase gene from a marine yeastPichia guilliermondiiand its expression inPichia pastoris

โœ Scribed by Tong Zhang; Fang Gong; Zhe Chi; Guanglei Liu; Zhenming Chi; Jun Sheng; Jing Li; Xianghong Wang


Publisher
Springer Netherlands
Year
2008
Tongue
English
Weight
819 KB
Volume
95
Category
Article
ISSN
0003-6072

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## Abstract An ovalbumin gene was cloned from Chinese quail (__Coturnix coturnix__) oviduct by RTโ€PCR and then inserted into the __P. pastoris__ genome under the control of the methanol inducible 5โ€ฒ alcohol oxidase (AOX) promoter. The recombinant __P. pastoris__ strain was demonstrated to be able t