✦ LIBER ✦

Molecular cloning and characterization of the dna of a new human papillomavirus (hpv 30) from a laryngeal carcinoma

✍ Scribed by Tomas Kahn; Elisabeth Schwarz; Harald Zur Hausen

Publisher: John Wiley and Sons
Year: 1986
Tongue: French
Weight: 522 KB
Volume: 37
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910370111

No coin nor oath required. For personal study only.

✦ Synopsis

DNA from a human laryngeal carcinoma was molecularly cloned in Lambda L47. The gene library was screened for human papillomavirus (HPV)-related sequences by hybridization analysis with 32P-labelled HPV 16 DNA at conditions of low stringency (Tm -40 degrees C). One of the clones (4-5) with an insert of 7.8 kb showed cross-hybridization with most of the known HPV types (Tm -40 degrees C), and with several of them even under more stringent conditions (Tm -30 degrees C). No signal was detected under high-stringency conditions (Tm -20 degrees C). The co-linear alignment of clone 4-5 with HPV 16 DNA could be demonstrated by hybridization experiments and also by partial DNA sequence analysis. We conclude that clone 4-5 represents a new HPV type tentatively designated HPV 30. HPV 30 DNA was also detected in 2 genital lesions but not in 41 laryngeal carcinomas analyzed so far. Its presence in other tumor DNA is now under investigation.

📜 SIMILAR VOLUMES

Characterization of a new human papillom

Characterization of a new human papillomavirus (HPV 41) from disseminated warts and detection of its DNA in some skin carcinomas

✍ M. Grimmel; E.-M. De Villiers; Ch. Neumann; M. Pawlita; H. zur Hausen 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 French ⚖ 476 KB

A new human papillomavirus type (HPV 41). distantly related to known HPV prototypes, was isolated from a patient with disseminated facial, peri-anal and foot warts (epidermodysplasia verruciformis was not diagnosed). The viral DNA was molecularly cloned in 2 BarnHl restriction fragments with sizes o

Molecular cloning and characterization o

Molecular cloning and characterization of a glucan synthase gene from the human pathogenic fungusParacoccidioides brasiliensis

✍ Pereira, Maristela; Felipe, M. Sueli S.; Br�gido, Marcelo M.; Soares, C�lia M. A 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 420 KB 👁 2 views

1,3-b-D-glucan is a fungal cell wall polymer synthesized by the multi-subunit enzyme 1,3-b-D-glucan synthase. A subunit of this integral membrane protein was ®rst described as the product of the FKS1 gene from Saccharomyces cerevisiae using echinocandin mutants. Other FKS1 genes were also reported f

Molecular cloning and analysis of a DNA

Molecular cloning and analysis of a DNA repetitive element from the mouse genome

✍ Adriana Geisinger; Gabriela Cossio; Rodolfo Wettstein 📂 Article 📅 2006 🏛 The American Society for Biochemistry and Molecula 🌐 English ⚖ 606 KB

Establishment and characterization of a

Establishment and characterization of a new cell line TC-YIK originating from argyrophil small cell carcinoma of the uterine cervix integrating HPV16 DNA

✍ Hiroshi Ichimura; Masato Yamasaki; Ikuo Tamura; Tetsuo Katsumoto; Masumi Sawada; 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 545 KB

A new cell line, designated TC-YIK, was established from YIK-1 tumor cells, derived from argyrophil small cell carcinoma (ASCC) of the uterine cervix, and serially heterotransplanted into nude mice, integrating human papillomavirus type 16 (HPV16) DNA. The population doubling time of TC-YIK was appr

Isolation, cloning, and characterization

Isolation, cloning, and characterization of the 2S albumin: A new allergen from hazelnut

✍ Cristiano Garino; Laurian Zuidmeer; Justin Marsh; Alison Lovegrove; Maria Morati 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 229 KB

## Abstract **Scope:** 2S albumins are the major allergens involved in severe food allergy to nuts, seeds, and legumes. We aimed to isolate, clone, and express 2S albumin from hazelnut and determine its allergenicity. **Methods:** 2S albumin from hazelnut extract was purified using size exclusion c

Cloning and characterization of a new is

Cloning and characterization of a new isoform of Choristoneura hormone receptor 3 from the spruce budworm

✍ Subba R. Palli; Tim R. Ladd; Arthur Retnakaran 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 147 KB 👁 2 views

Choristoneura hormone receptor 3 (CHR3) is a 20E (20-hydroxyecdysone)induced delayed early gene that is homologous to Manduca hormone receptor 3 (MHR3), Drosophila hormone receptor 3 (DHR3), and Galleria hormone receptor 3 (GHR3). We recently cloned and characterized a cDNA that was copied from the