Establishment and characterization of a new cell line TC-YIK originating from argyrophil small cell carcinoma of the uterine cervix integrating HPV16 DNA
✍ Scribed by Hiroshi Ichimura; Masato Yamasaki; Ikuo Tamura; Tetsuo Katsumoto; Masumi Sawada; Osamu Kurimura; Jun-Ichi Furuyama; Takashi Kurimura
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 545 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
A new cell line, designated TC-YIK, was established from YIK-1 tumor cells, derived from argyrophil small cell carcinoma (ASCC) of the uterine cervix, and serially heterotransplanted into nude mice, integrating human papillomavirus type 16 (HPV16) DNA. The population doubling time of TC-YIK was approximately 21.6 hours at the 119th subculture. Subcutaneous injection of 1 x 10' TC-YIK cells into nude mice yielded a solid tumor. The cytologic appearance of TC-YIK was similar to that of YIK-1. The TC-YIK cells contained argyrophil granules and neurosecretory granules in the cytoplasm and showed positive immunohistochemical staining for neuron-specific enolase, serotonin, and chromogranin. Thus, TC-YIK retained the histochemical characteristics of ASCC. The TC-YIK cells contained HPV16 DNA in a multiple-copy integrated form and actively transcribed the integrated HPV16 genome. Amplification of the c-myc oncogene was observed in the TC-YIK cells. These data suggest that TC-YIK is a useful in vitro experimental model of ASCC and that HPVl6 and c-myc may play some role in the genesis of this malignant tumor and/or maintenance of the transformed TC-YIK phenotype. Cancer 67: 2327-2332,1991. RGYROPHIL SMALL CELL CARCINOMA (ASCC) of the A uterine cervix, first described by Albores-Saavedra and co-workers' in 1972, is considered to be derived from the amine precursor uptake and decarboxylation cell sys-It is characterized pathologically by the presence of argyrophil granules in the cytoplasm of the undifferentiated small cells and clinically by rapid metastasis and a poor p r o g n ~s i s . ~-~ Because ASCC is a rare cervical tumor. accounting for only 0.5% to 5% of all cases,'.' most reports on ASCC have been concerned with single cases or small series. Therefore, development of in vivo and in