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Molecular Cloning and Characterization of cDNA Encoding the GTP-Binding Protein αiand Identification of a Related Protein, αh

✍ Scribed by Thomas Michel, John W. Winslow, John A. Smith, J. G. Seidman and Eva J. Neer

Book ID
123633242
Publisher
National Academy of Sciences
Year
1986
Tongue
English
Weight
842 KB
Volume
83
Category
Article
ISSN
0027-8424

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✍ Colette Jako; Bernard Teyssendier Serve 📂 Article 📅 1996 🏛 Springer 🌐 English ⚖ 388 KB

The cloning of small GTP-binding proteins from Petunia hybrida was performed using a PCR-based strategy. Degenerate primers were designed from the DTAGQE and FMETSA consensus sequences. Three different cDNAs were amplified. The deduced polypeptide sequences PhPCRGP1 and PhPCRGP2 were homologous to R