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Characterization of cDNA and genomic DNA encoding the a subunit of a human GTP-binding protein Gi

✍ Scribed by Sunyoung Kim; Siew-Lan Ang; Richard Lee; Yasuhiro Kawahara; J.G. Seidman; Eva J. Neer

Book ID
119442778
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
69 KB
Volume
19
Category
Article
ISSN
0022-2828

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The cloning of small GTP-binding proteins from Petunia hybrida was performed using a PCR-based strategy. Degenerate primers were designed from the DTAGQE and FMETSA consensus sequences. Three different cDNAs were amplified. The deduced polypeptide sequences PhPCRGP1 and PhPCRGP2 were homologous to R