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Molecular arrangement of DNA in bacteriophage T4

✍ Scribed by Samarendra Basu

Publisher: Wiley (John Wiley & Sons)
Year: 1977
Tongue: English
Weight: 973 KB
Volume: 16
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1977.360161015

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✦ Synopsis

Abstract

The degree of preferred orientation and the coiling of the deoxyribonucleic acid within phage T~4~ was studied by two independent techniques, namely, polarization of fluorescence and uv linear dichroism. A correlation between the two kinds of data was obtained, which indicated that a significant proportion (about 30%) of total phage DNA is aligned preferentially along the long axis of phage heads. Analyses of the data suggest that all of the phage DNA cannot be in a highly supercoiled helical configuration. A few models of the DNA arrangement in T~4~ have been discussed in which linear sidewise packings of DNA would be predominant and may explain the observed longitudinal orientation of intraphage DNA.

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