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Molecular Analysis of the Breast Cancer Genes BRCA1 and BRCA2 Using Amplicon-Based Massive Parallel Pyrosequencing

✍ Scribed by Michils, Geneviève; Hollants, Silke; Dehaspe, Luc; Van Houdt, Jeroen; Bidet, Yannick; Uhrhammer, Nancy; Bignon, Yves-Jean; Vermeesch, Joris R.; Cuppens, Harry; Matthijs, Gert

Book ID
120008858
Publisher
American Society for Investigative Pathology
Year
2012
Tongue
English
Weight
941 KB
Volume
14
Category
Article
ISSN
1525-1578

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This study describes how the new massive parallel sequencing technology can be implemented in a diagnostic setting for the breast cancer susceptibility genes (BRCA1 and BRCA2). The throughput was maximized by increasing uniformity in coverage, obtained by a multiplex approach, which outperformed poo