In our hands, we occasionally observed negative-going reconstructed size-exclusion chromatograms in SEC/ESI-MS which are possibly related to polymer type, salt concentration and/or other operating conditions. In the IcniM?.
Molar-Mass Characterization of Cationic Polymers for Gene Delivery by Aqueous Size-Exclusion Chromatography
β Scribed by Xulin Jiang; Aschwin van der Horst; Mies J. van Steenbergen; Niels Akeroyd; Cornelus F. van Nostrum; Peter J. Schoenmakers; Wim E. Hennink
- Book ID
- 106493046
- Publisher
- Springer US
- Year
- 2006
- Tongue
- English
- Weight
- 540 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0724-8741
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## Abstract A systematic study of the effects of band broadening (BB) in sizeβexclusion chromatography (SEC) on Poisson molarβmass distributions (MMDs) is carried out. Such distributions apply for polymer standards used for SEC calibration, and they are a good model for the MMD from any quasiβlivin
Synthetic polymers having a polydispersity greater than 1.1 cannot be reliably characterized by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry alone. This complication has been overcome by off-line coupling of size-exclusion chromatography (SEC) with MALDI. Data