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Modulation of the phosphate-starvation response in Escherichia coli by genetic manipulation of the polyphosphate pathways

✍ Scribed by Susan T. Sharfstein; Stephen J. van Dien; J. D. Keasling

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 560 KB
Volume: 51
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(19960820)51:4<434::aid-bit6>3.0.co;2-j

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✦ Synopsis

The effect of intracellular polyphosphate on the phosphate-starvation response in Escherichia coliwas studied by genetically manipulating the intracellular polyphosphate levels and by performing phosphate shifts on the genetically engineered strains. Strains that produced large quantities of polyphosphate and were able to degrade it induced the phosphate-starvation response to a lesser extent than wild-type strains, whereas strains that were unable to degrade a large intracellular polyphosphate pool induced the phosphate-starvation response to a greater extent than wild-type strains. These results have important implications for expression of heterologous genes under control of the phoA promoter. 0 1996

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