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In silico prediction and validation of the importance of the Entner–Doudoroff pathway in poly(3-hydroxybutyrate) production by metabolically engineered Escherichia coli

✍ Scribed by Soon Ho Hong; Si Jae Park; Soo Yun Moon; Jong Pil Park; Sang Yup Lee


Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
547 KB
Volume
83
Category
Article
ISSN
0006-3592

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A recombinant Escherichia coli strain XL1-Blue harboring a stable high-copy-number plasmid pSYL107 containing the Alcaligenes eutrophus polyhydroxyalkanoate biosynthesis genes and the Escherichia coli ftsZ gene was employed for the production of poly(3hydroxybutyrate) (PHB) by fed-batch culture in a