Modulation of the catabolic effects of interleukin-1β on human articular chondrocytes by transforming growth factor-β

✍ Scribed by Zhao-Ping Lum; Brian E. Hakala; John S. Mort; Anneliese D. Recklies

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 741 KB
• Volume: 166
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/(sici)1097-4652(199602)166:2<351::aid-jcp13>3.0.co;2-e

✦ Synopsis

The effects of IL-1 p and TGF-P on the biosynthesis of extracellular matrix structural components relative to the metalloproteinases and their inhibitor TlMPl in human articular chondrocytes were investigated. It has been proposed that TGFp, acting as a positive regulator of matrix accretion, can counteract the increased loss of cartilage matrix induced by IL-1 p. To allow a comparison of their effects on mRNA levels for these different components, quantitation by competitive RT/ PCR was employed. This method was found to give reproducible estimates of mRNA levels and the observed effects of IL-1 p and TGF-j3 on individual compo-