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Transforming Growth Factor β1 Regulates Tissue Inhibitor of Metalloproteinases—1 Expression in Differentiated Human Articular Chondrocytes

✍ Scribed by Monika Günther; Hans-Dieter Haubeck; Eddy Van De Leur; Jörg Bläser; Sven Bender; Ines Gütgemann; Dagmar-Christiane Fischer; Harald Tschesche; Helmut Greiling; Peter C. Heinrich; Lutz Graeve

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
972 KB
Volume
37
Category
Article
ISSN
0004-3591

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✦ Synopsis

Objective. To investigate the role of interleukin-6 (IL-6) and transforming growth factor pl (TGFp1) in the regulation of tissue inhibitor of metalloproteinases-1 (TIMP-1) synthesis in human articular chondrocytes.

Methods. Articular cartilage was obtained from human knee joints 24 hours after death. Chondrocytes were isolated by collagenase digestion and embedded in low-gellingtemperature agarose. After stimulation by cytokines, total RNA was isolated and analyzed by Northern blotting. TIMP-1 protein levels were determined using a competitive enzyme-linked immunosorbent assay.

Results. Human chondrocytes in agarose culture expressed messenger RNA (mRNA) for the IL-6 receptor (gp80) and its signal-transducing subunit gp130. In contrast to the findings in a previous study, IL-6 did not stimulate TIMP-1 expression in these cells, whereas TGFPl was an important inducer of TIMP-1 mRNA and protein synthesis.

Conclusion. Our findings suggest that TGFBl

Supported by grants from the Deutsche Forschungsanstalt fiir Luft-und Raumfahrt, Bundesministerium fur Forschung und Technologie, the Deutsche Forschungsgemeinschaft, and the Fonds der Chemischen Industrie.

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