Modulation of DNA-binding domains for sequence-specific DNA recognition
✍ Scribed by Ronen Marmorstein; Mary X. Fitzgerald
- Book ID
- 117521221
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 748 KB
- Volume
- 304
- Category
- Article
- ISSN
- 0378-1119
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Double-stranded DNA can be viewed as a multifunctional, modular receptor that can be read sequenceselectively in a digital way (base pair per base pair) by a complementary, similarly modular ligand. This principle has been exploited in several approaches to design sequence-specific DNA-binding ligan
## Abstract __Trp__ repressor (25 kDa) is a regulatory protein that controls transcription initiation in the tryptophan biosynthetic operon and at least four other operons in __Escherichia coli__. An alanine to valine mutation (AV77) in the DNA binding domain is known to increase repressor activity