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Development of a protein microarray using sequence-specific DNA binding domain on DNA chip surface

โœ Scribed by Yoo Seong Choi; Seung Pil Pack; Young Je Yoo

Book ID
116290024
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
377 KB
Volume
329
Category
Article
ISSN
0006-291X

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Affinity chromatography of a sequence-sp
Affinity chromatography of a sequence-specific DNA binding protein using teflon-linked oligonucleotides
โœ Craig H. Duncan; Sharon L. Cavalier ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 594 KB

An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated o