𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Affinity chromatography of a sequence-specific DNA binding protein using teflon-linked oligonucleotides

✍ Scribed by Craig H. Duncan; Sharon L. Cavalier

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
594 KB
Volume
169
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated on an automated DNA synthesizer, contains high levels of attached DNA, and has superior mechanical properties. It should be generally useful for affinity chromatography of DNA binding proteins.

πŸ“œ SIMILAR VOLUMES

Selection of High-Affinity Binding Sites
Selection of High-Affinity Binding Sites for Sequence-Specific, DNA Binding Proteins from Random Sequence Oligonucleotides
✍ S. Pierrou; S. Enerback; P. Carlsson πŸ“‚ Article πŸ“… 1995 πŸ› Elsevier Science 🌐 English βš– 695 KB

We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble

A New Analytical Scale DNA Affinity Bind
A New Analytical Scale DNA Affinity Binding Assay for Analyses of Specific Protein–DNA Interactions
✍ N. Vinay Kumar; Lori R. Bernstein πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 160 KB

We describe a rapid analytical assay for identification of proteins binding to specific DNA sequences. The DAPSTER assay (DNA affinity preincubation specificity test of recognition assay) is a DNA affinity chromatography-based microassay that can discriminate between specific and nonspecific protein

Specific and efficient targeting of aden
Specific and efficient targeting of adenovirus vectors to macrophages: application of a fusion protein between an adenovirus-binding fragment and avidin, linked to a biotinylated oligonucleotide
✍ J. C. Emile Gras; Paul Verkuijlen; Rune R. Frants; Louis M. Havekes; Theo J. C. πŸ“‚ Article πŸ“… 2006 πŸ› John Wiley and Sons 🌐 English βš– 704 KB

## Abstract ## Background The application of serotype 5 adenoviruses (Ad5) in macrophages is hampered by the absence of the endogenous coxsackie adenovirus receptor (CAR). ## Methods To overcome this limitation, we first generated a linker protein consisting of the virus‐binding domain of CAR an