✦ LIBER ✦

Modulation of Cell Cycle for Enhancement of Antibody Productivity in Perfusion Culture of NS0 Cells

✍ Scribed by Neysi Ibarra; Shikiko Watanabe; Jin-Xiu Bi; John Shuttleworth; Mohamed Al-Rubeai

Book ID: 109386622
Publisher: American Institute of Chemical Engineers
Year: 2003
Tongue: English
Weight: 91 KB
Volume: 19
Category: Article
ISSN: 8756-7938
DOI: 10.1021/bp025589f

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Suppression of apoptosis in perfusion cu

Suppression of apoptosis in perfusion culture of Myeloma NS0 cells enhances cell growth but reduces antibody productivity

✍ B. T. Tey; M. Al-Rubeai 📂 Article 📅 2004 🏛 Springer 🌐 English ⚖ 284 KB

Cell cycle kinetics and monoclonal antib

Cell cycle kinetics and monoclonal antibody productivity of hybridoma cells during perfusion culture

✍ Sun Ho Park; Dewey D. Y. Ryu 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 565 KB

## Abstract The flow‐cytometric (FCM) analysis of bivariate DNA/lgG distributions has been conducted to study the cell cycle kinetics and monoclonal antibody (MAb) production during perfusion culture of hybridoma cells. Three different perfusion rates were employed to demonstrate the dependency of

Effect of Bcl-2 overexpression on cell c

Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells

✍ Beng Ti Tey; Mohamed Al-Rubeai 📂 Article 📅 2005 🏛 Elsevier Science 🌐 English ⚖ 420 KB

Detailed understanding of enhanced speci

Detailed understanding of enhanced specific antibody productivity in NS0 myeloma cells

✍ Soo Hean Gary Khoo; Mohamed Al-Rubeai 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 398 KB 👁 1 views

## Abstract The understanding of how cellular productivity is modulated in cell lines is of significant importance in the biopharmaceutical industry. Often, single molecular mechanisms fail to fully explain how specific antibody productivity is enhanced during proliferation arrest. Previously, we r

Perfusion Cultures of Hybridoma Cells fo

Perfusion Cultures of Hybridoma Cells for Monoclonal Antibody Production

✍ FRANCISCO J. CASTILLO; LARRY J. MULLEN; JOHN C. THRIFT; BARRY C. GRANT 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 393 KB

Fed-batch culture of recombinant NS0 mye

Fed-batch culture of recombinant NS0 myeloma cells with high monoclonal antibody production

✍ Weichang Zhou; Chun-Chiang Chen; Barry Buckland; John Aunins 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 231 KB 👁 2 views

An amplified NS0 cell line transfected with a vector expressing a humanized monoclonal antibody (MAb) against CD-18 and glutamine synthetase (GS) was cultivated in a 1.5 L fed-batch culture using a serum-free, glutamine-free medium. Concentrated solutions of key nutrient components were fed periodic