Milk-derived growth factors as serum supplements for the growth of fibroblast and epithelial cells
โ Scribed by David A. Belford; Mary-Louise Rogers; Geoffrey O. Regester; Geoffrey L. Francis; Geoffrey W. Smithers; Ingrid J. Liepe; Ilka K. Priebe; F. John Ballard
- Book ID
- 112803497
- Publisher
- Society for In Vitro Biology
- Year
- 1995
- Tongue
- English
- Weight
- 1016 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1475-2689
No coin nor oath required. For personal study only.
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Mitogenic effects of fetal calf serum (FCS), plateletderived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor-p (TGF-P), and forskolin to adult mouse Schwann cells were examined by bromodeoxyuridine (BrdU) incorporation and double immunofluorescence for SlOO and BrdU.
Two batches of experimental media containing 10% milk whey (sweet or sour) and 1% bovine serum supported the growth of BHK-21 cells in serial passages. The cell yield was greater than 2 x 10(6)/ml at 48 h. The cells were susceptible to inoculation with foot and mouth disease (FMD) virus type 'A' and