✦ LIBER ✦

Milk whey as a serum supplement for the growth of FMD virus in BHK-21 cells

✍ Scribed by S.N. Saha; A.K. Sen

Publisher: Springer Netherlands
Year: 1989
Tongue: English
Weight: 240 KB
Volume: 13
Category: Article
ISSN: 0165-7380
DOI: 10.1007/bf00346718

No coin nor oath required. For personal study only.

✦ Synopsis

Two batches of experimental media containing 10% milk whey (sweet or sour) and 1% bovine serum supported the growth of BHK-21 cells in serial passages. The cell yield was greater than 2 x 10(6)/ml at 48 h. The cells were susceptible to inoculation with foot and mouth disease (FMD) virus type 'A' and the virus titres obtained were comparable with those in Eagle's MEM-G with 10% serum. The use of milk whey could reduce the consumption of serum required for the growth of FMD virus in BHK-21 cells by up to 90%.

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