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Mitogenic effects of platelet-derived growth factor, fibroblast growth factor, transforming growth factor-β, and heparin-binding serum factor for adult mouse Schwann cells

✍ Scribed by K. Watabe; T. Fukuda; J. Tanaka; K. Toyohara; O. Sakai

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
956 KB
Volume
39
Category
Article
ISSN
0360-4012

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✦ Synopsis

Mitogenic effects of fetal calf serum (FCS), plateletderived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor-p (TGF-P), and forskolin to adult mouse Schwann cells were examined by bromodeoxyuridine (BrdU) incorporation and double immunofluorescence for SlOO and BrdU. PDGF-BB, basic FGF, and TGF-PI and f32 were all mitogenic for Schwann cells in media containing FCS. Forskolin suppressed the mitogenic activity of these factors. In serum-free media, PDGF-BB and bFGF were also mitogenic, but TGF-p1 and p2 were not. Heparin-binding fractions of FCS obtained by heparin-Sepharose chromatography synergized with TGF-pl and p2 to produce a mitogenic response. Since PDGF-BB, acidic FGF, and basic FGF were not detected in these fractions by immunoabsorption and immunoblot assays, the presence of unidentified heparin-binding molecules in FCS bioactive for adult mouse Schwann cells is suggested.

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