Insulin-like growth factor I receptor is required for the mitogenic and transforming activities of the platelet-derived growth factor receptor

✍ Scribed by Tiziana Deangelis; Andres Ferber; Renato Baserga

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 971 KB
• Volume: 164
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041640126

✦ Synopsis

Abstract

R^−^ cells are 3T3‐like cells derived from mouse embryos in which the insulin‐like growth factor I (IGF‐I) receptor (IGF‐IR) genes have been disrupted by targeted homologous recombination. These cells cannot grow in serum‐free medium supplemented by the growth factors that sustain the growth of other 3T3 cell lines, and cannot be transformed by oncogenes that easily transform wild type mouse embryo cells. We have used these cells to study the role of the IGF‐IR in the growth and transformation of cells overexpressing the platelet‐derived growth factor (PDGF)‐b̃b̃ receptor. We report that an overexpressed PDGF‐b̃b̃ receptor fails to induce mitogenesis or transformation in cells lacking the IGF‐IR, while capable of doing so in cells expressing the IGF‐IR. We conclude that the ability of the activated PDGF‐b̃b̃ receptor to stimulate cell proliferation and transformation requires a funcitional IGF‐IR. © 1995 Wiley‐Liss, Inc.