Microwave-assisted extraction and determination of cyanuric acid residue in pet food samples by liquid chromatography–tandem mass spectrometry

✍ Scribed by Chao Han; Yongfang Zhou; Biqi Xia; Zhenou Zhu; Cuiping Liu; Yan Shen

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 108 KB
• Volume: 34
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201000761

✦ Synopsis

Abstract

Cyanuric acid (CYA) is attracting more attention due to its potential toxicity. In the present work, microwave‐assisted extraction method in combination with liquid chromatography–tandem mass spectrometry (LC‐MS/MS) was proposed for the determination of CYA in pet food samples. Among different solvents, diethylamine–acetonitrile–water mixture (1:5:4, v/v) was found to be the best one as the extractant due to the strong polarity of CYA in the pet food. An internal standard, ^13^C~3~‐labeled CYA, was used in the extractions. The separation was performed on a MERCK ZIC HILIC column (150 mm×2.1 mm id, 5 μm) with gradient elution of 20 mM ammonium acetate solution–acetonitrile. CYA was well retained (Rt=5.10 min) and eluted with good peak shape. The method could respond linearly with CYA at concentrations from 1.0 to 50 ng/mL with a quantification limit of 0.25 mg/kg. The intra‐ and inter‐day precision was less than 4.0% and the recovery of the assay was in the range of 90.4–108.1%. In the analysis of practical spiked pet food samples, the new method yielded satisfactory results. Due to its simplicity and accuracy the straightforward method is particularly suitable for routine CYA detection.