Conformational rate processes in aqueous solutions of uniformly ~SN-labeled pancreatic trypsin inhibitor (BPTI) at 36 ~ were investigated by measuring the rotating frame relaxation times of the backbone 15N spins as a function of the spin-lock power. Two different intramolecular exchange processes w
Microsecond Protein Dynamics Measured by 13Cα Rotating-Frame Spin Relaxation
✍ Scribed by Patrik Lundström; Mikael Akke
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 162 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1439-4227
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The '"C off-resonance rotating frame spin-lattice relaxation technique is applicable to the study of protein rotational diffusion behavior in both model in vitro and in vivo systems. The original formalism of James and co-workers [(1978) J. Am. Chem. SOC. 100, 3590-35941 was constrained by the assum
13CC(-13C0 homonuclear NOE and 13CO T~ relaxation were measured for a 20 kDa protein using tripleresonance pulse sequences. The experiments were sufficiently sensitive to obtain statistically significant differences in relaxation parameters over the molecule. The 13C%13C0 cross-relaxation rate, obta
## Abstract The ^13^C off‐resonance rotating frame spin‐lattice relaxation technique is applicable to the study of protien rotational diffusion behaviour in a variety of experimental situations. The original formalism of James and co‐workers (1978) (__J. Amer. Chem. Soc.__ 100, 3590–3594) was const