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Study of protein dynamics in solution by measurement of13Cα-13CO NOE and13CO longitudinal relaxation

✍ Scribed by Lei Zeng; Mark W. F. Fischer; Erik R. P. Zuiderweg

Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
464 KB
Volume
7
Category
Article
ISSN
0925-2738

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✦ Synopsis

13CC(-13C0 homonuclear NOE and 13CO T~ relaxation were measured for a 20 kDa protein using tripleresonance pulse sequences. The experiments were sufficiently sensitive to obtain statistically significant differences in relaxation parameters over the molecule. The 13C%13C0 cross-relaxation rate, obtained from these data, is directly proportional to an order parameter describing local motion and it is largely independent of the local correlation time. It is therefore a relatively straightforward observable for the identification of local dynamics.

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