Assesment of protein reorientational diffusion in solution by 13C off-resonance rotating frame spin–lattice relaxation: Effect of polydispersity

✍ Scribed by Courtney F. Morgan; Thomas Schiliech; G. Herbert Caines

• Publisher: Wiley (John Wiley & Sons)
• Year: 1990
• Tongue: English
• Weight: 482 KB
• Volume: 29
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.360290305

✦ Synopsis

The '"C off-resonance rotating frame spin-lattice relaxation technique is applicable to the study of protein rotational diffusion behavior in both model in vitro and in vivo systems. The original formalism of James and co-workers [(1978) J. Am. Chem. SOC. 100, 3590-35941 was constrained by the assumption of random isotropic reorientational motion of a monodisperse protein population. Here we extend the formalism to include polydispersity. Application is made to the alkaline pH induced association of lysozyme, lysozyme-bovine serum albumin mixtures, and to the phase separation of lysozyme salt-water mixtures induced by low temperature.