Microparticle-enhanced nephelometric immunoassay of human plasminogen

✍ Scribed by E. Marchal; P. Montagne; M. L. Cuillière; M. C. Béné; G. Faure

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 538 KB
• Volume: 10
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/(sici)1098-2825(1996)10:2<85::aid-jcla5>3.0.co;2-k

✦ Synopsis

A microparticle-enhanced nephelometric immunoassay was developed for plasminogen quantitation in human plasma. It is based on the nephelometric measurement of the light scattered by microparticle clusters formed during a sandwich reaction between plasminogen, microparticle-anti-plasmino- gen conjugate, and the free antibodies of anti-plasminogen rabbit antiserum. This immunoassay was sensitive (detection limit in reaction mixture, 3 4 ~g / L ) and could be performed in 500-fold diluted human plasma, excluding any interference or sample pretreatment. It allowed the quantitation of plasminogen on a large range of concentrations (1 7-550 mg/L), with a security in antigen excess reaching 1,100 mg/L, with accuracy (linear recovery in dilution-overloading assay and correlation with conventional immunonephelometry), and precision (within-and between-run coefficients of variation lower than 8%).A normal reference range from 54 to 148 mg/L (mean 2 2 SD) was calculated from plasminogen concentration in plasma from 130 adults. Easy to perform (no washing or phase separation) and rapid (two steps of 30 minutes then 1 hour of incubation at room temperature), this microparticle-enhanced nephelometric immunoassay could be an interesting alternative method for human plasminogen quantitation. o 1996 Wiley-Liss, Inc.