Micromethods for Protein Structure Analysis
β Scribed by John E. Shively
- Book ID
- 115638562
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 640 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1046-2023
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π SIMILAR VOLUMES
A simple and nondestructive method was developed for effective removal of lipids, such as long-chain fatty acids and steroids, from small quantities \((2-10 \mathrm{mg}\) ) of aqueous protein. The procedure operates with a high recovery of protein \((97 \%)\) and was elaborated by using different al
Reaction of erythrosin B with proteins results in a stable, highly colored chromophore with an absorbance maximum at \(545 \mathrm{~nm}\). This is the basis for a new quantitative determination method of proteins in solution. The assay can be performed at room temperature, but is faster and more sen